The Anopheles gambiae M Mali-NIH colony was established from blood-fed adult females collected resting inside houses in Niono, Mali in November 2005. Approximately 80 isofemale families molecularly identified as An. gambiae M form were used to establish the colony, which was subsequently karyotyped as 2Rbc/bc; 2La/a. The A. gambiae M Mali-NIH colony is currently maintained at MR4.


Anopheles coluzzii, formerly known as Anopheles gambiae M molecular form, was defined as a separate species in 2013 (Coetzee et al.). An. coluzzii belongs to the Anopheles gambiae species complex, which consists of at least seven species.

Genome assemblies and gene sets