Web:C. pipiens Homepage

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question_32.png C. pipiens Homepage
Go to the C. pipiens quinquefasciatus homepage.

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[edit] Culex pipiens

Culex pipiens is a mosquito species which acts as a vector for West Nile virus, Lymphatic filariasis, Japanese encephalitis, St. Louis encephalitis.

[edit] Source of the Johannesburg strain used in sequencing

The Johannesburg (JHB) strain is the source of genomic DNA for the Culex pipiens quinquefasciatus genome project. This strain has been selected because it consistently yields high quality chromosome spreads suitable for in situ hybridization studies [1]. This strain is also insecticide susceptible and so can be transported and maintained in laboratories world-wide. The Johannesburg (JHB) strain was established from offspring reared from 20 egg rafts collected in a single unused fish pond in March 2001. Population genetic studies revealed that the Johannesburg C. pipiens quinquefasciatus population was genetically isolated from a sympatric C. pipiens pipiens population [2]. Consequently, the JHB strain is expected to possess a high degree of genetic divergence from C. pipiens pipiens. Based on many generations of inbreeding at relatively small population size (often no more than about 50 females per generation), the colony is expected to show relatively little genetic variation [3]. In addition to having excellent polytene chromosomes in larval salivary gland tissues, this colony has also been found to be free of any visible polytene chromosome inversions.

[edit] Genome

The Culex pipiens JHB genome is approximately 540 Mb in length with a GC content of 37.4%.

Data related to the genome project have been deposited in GenBank under the project accession: AAWU01000000.

[edit] References

  1. ↑ A.J. Cornel, personal communication
  2. ↑ Cornel AJ, McAbee RD, Rasgon J, Stanich MA, Scott TW and Coetzee M. (2003). Differences in extent of genetic introgression between sympatric Culex pipiens and Culex quinquefasciatus (Diptera: Culicidae) in California and South Africa. J. Med. Entomol. 40: 36-51 PMID: 12597651
  3. ↑ A.J. Cornel, personal communication


[edit] Links to VectorBase site


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